The hematological improvement of rainbow trout (Oncorhynchus mykiss) during dietary supplementation with vitamin C after exposure to zinc nano-particles

The aim of this study was to examine the adverse effects of zinc nanoparticles on hematological indices of trout and investigate the improvement of these indices after vitamin C treatments. This study assesses the protective role of vitamin C in fish exposed to ZnO NPs. Two concentrations of ZnO-NPs (40 and 80 mg L-1) and two doses of vitamin C (400 and 800 mg per kg of feed) were used to treat 162 specimens of Oncorhynchus mykiss. No mortality was observed during the test. After 5 and 10 days of exposure, hematological data were analyzed according to routine clinical methods. Statistical analysis showed significant changes in WBCs and RBC on day 10 (p < /em>>0.05), while significant increase in neutrophils and monocytes, and decrease in lymphocyte cells were recorded (p < /em>